@article{158311,
  author = {Wen Xi Cao and Sarah Kabelitz and Meera Gupta and Eyan Yeung and Sichun Lin and Christiane Rammelt and Christian Ihling and Filip Pekovic and Timothy C. H. Low and Najeeb U. Siddiqui and Matthew H. K. Cheng and Stephane Angers and Craig A. Smibert and Martin W{\"u}hr and Elmar Wahle and Howard Lipshitz},
  title = {Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition},
  abstract = { In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2\% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). While the ubiquitin-proteasome system is necessary for clearance of all four repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL and ME31B for degradation early in the MZT; the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG makes the protein immune to degradation. We show that artificially persistent SMG downregulates the zygotic re-expression of mRNAs whose maternal contribution is cleared by SMG. Thus, clearance of SMG permits an orderly MZT. },
  year = {2019},
  journal = {bioRxiv},
  pages = {862490},
  url = {https://www.biorxiv.org/content/biorxiv/early/2019/12/03/862490.full.pdf},
  doi = {10.1101/862490},
  language = {eng},
}