@article{204356,
  author = {Moshe Kafri and Weronika Patena and Lance Martin and Lianyong Wang and Gillian Gomer and Arthur Sirkejyan and Audrey Goh and Alexandra Wilson and Sophia Gavrilenko and Michal Breker and Asael Roichman and Claire McWhite and Joshua Rabinowitz and Frederick Cross and Martin W{\"u}hr and Martin Jonikas},
  title = {Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery},
  abstract = {<p>Photosynthesis is central to food production and the Earth{\textquoteright}s biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii, we identify with high confidence (FDR\&lt;0.11) 70 previously-uncharacterized genes required for photosynthesis. We then provide a resource of mutant proteomes that enables functional characterization of these novel genes by revealing their relationship to known genes. The data allow assignment of 34 novel genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Additional analysis uncovers at least seven novel critical regulatory proteins, including five Photosystem I mRNA maturation factors and two master regulators: MTF1, which impacts chloroplast gene expression directly; and PMR1, which impacts expression via nuclear-expressed factors. Our work provides a rich resource identifying novel regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.Competing Interest StatementThe authors have declared no competing interest.</p>
},
  year = {2022},
  journal = {bioRxiv},
  publisher = {Cold Spring Harbor Laboratory},
  url = {https://www.biorxiv.org/content/early/2022/11/15/2022.11.12.515357},
  doi = {10.1101/2022.11.12.515357},
}