@article{204381,
  keywords = {photosynthesis, photosystem I maturation factors, methionyl-tRNA formyltransferase, retrograde regulation, genetics, protein profiling, RNA sequencing, protein localization},
  author = {Moshe Kafri and Weronika Patena and Lance Martin and Lianyong Wang and Gillian Gomer and Sabrina Ergun and Arthur Sirkejyan and Audrey Goh and Alexandra Wilson and Sophia Gavrilenko and Michal Breker and Asael Roichman and Claire McWhite and Joshua Rabinowitz and Frederick Cross and Martin W{\"u}hr and Martin Jonikas},
  title = {Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery},
  abstract = {<p>Summary Photosynthesis is central to food production and the Earth{\textquoteright}s biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii, we identify with high confidence (false discovery rate [FDR]\&nbsp;\&lt;\&nbsp;0.11) 70 poorly characterized genes required for photosynthesis. We then enable the functional characterization of these genes by providing a resource of proteomes of mutant strains, each lacking one of these genes. The data allow assignment of 34 genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Further analysis uncovers biogenesis/regulatory roles for at least seven proteins, including five photosystem I mRNA maturation factors, the chloroplast translation factor MTF1, and the master regulator PMR1, which regulates chloroplast genes via nuclear-expressed factors. Our work provides a rich resource identifying regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.</p>
},
  year = {2023},
  journal = {Cell},
  volume = {186},
  number = {25},
  pages = {5638-5655},
  issn = {0092-8674},
  url = {https://www.sciencedirect.com/science/article/pii/S0092867423012242},
  doi = {10.1016/j.cell.2023.11.007},
}